1 human embryonic stem cell derivation
Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
Ngày tải lên :
10/08/2014, 05:20
... Marshall VS, Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from ... from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells ... enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types of cardiac...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... Microtubule-associated protein MEF Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the ... ………………………………………………………… 1.1 Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded ... NANOG and SOX2 constitute the human embryonic stem cell transcriptional core The transcription factors OCT4, NANOG and SOX2 play essential roles in early embryonic development, and are among...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL ... Glial progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes simplex virus thymidine kinase Interferon Interleukin Induced pluripotent stem Luria broth Leukemia inhibitory ... deciphering the mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell-derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
Ngày tải lên :
11/09/2015, 10:06
... replaced by transplant-friendly and costeffective culture systems In view of the imperfections of existing culture systems, an optimal system for the culture and derivation of hESCs would require ... the culture of MEF, albeit in a separate system, for conditioned medium As stated earlier, one of the problems with large scale expansion using feeder systems that require MEFs is the limited culture ... currently no data supporting hESC proliferation on this material 1.4 Ideal hESC Culture System The various culture systems described above have sought to improve the culture of hESCs, but there are still...
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Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) ... reserved Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 16 Guidelines for Human Embryonic Stem Cell Research team derived stem cells from primordial gonadal...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... bp: base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: ... distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative analysis of ... browser, Amander Clark for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research at UCLA SF is Special...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
Ngày tải lên :
14/08/2014, 08:20
... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... promote hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell-derived NK cells acquire functional ... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... a model system (Dinger et al., 2008; Guttman et al., 2011; Mercer et al., 2010; Sheik Mohamed et al., 2010; Tochitani and Hayashizaki, 2008) To date, the roles of lncRNAs in human embryonic and ... neuronal differentiation respectively 82 Chapter VI – Long Non-coding RNAs Regulate Human Embryonic Stem Cell Pluripotency 6.1 Introduction LncRNAs are integral members of the ESC self-renewal ... pluripotent cell state The studies on lncRNAs and pluripotency were all performed in mouse embryonic stem cells (mESCs) Results from the mouse studies may not be directly applicable to hESCs,...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated a REST binding peak upstream of RMST, ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST ... neurogenesis upon RMST knockdown in ReN-VM neural stem cells (Figures 7.4, 7.5 and 8.7) Knockdown of RMST appeared to shut down the neurogenic program of neural stem cells, with the cells adopting a glia...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, ... H.H., and Choo, A.B (2009) KLF4 and PBX1 directly regulate NANOG expression in human embryonic stem cells Stem Cells 27, 2114-2125 Chen, G., Yin, K., Shi, L., Fang, Y., Qi, Y., Li, P., Luo, J., ... model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic stem cells...
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Human embryonic stem cell derivatives as cancer therapeutics
Ngày tải lên :
09/09/2015, 18:52
... protein Embryonic stem cell Forward scatter -Galactosylceramide Granulocyte-macrophage colony-stimulating factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic ... dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic progenitor cell Hematopoietic stem cell Herpes simplex virus ... observed 1.2 Adult stem cells and cancer therapy Stem cells, by definition, possess two main characteristics Stem cells are able to produce daughter cells that retain their stem cell potential...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
Ngày tải lên :
11/09/2015, 10:06
... culture medium, mTeSR-1, which has been optimized for Matrigel, the standard feeder-free culture system Subculture was done every 5-7 days depending on the confluency of the hESCs In this way, hESCs...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
Ngày tải lên :
11/09/2015, 10:07
... aging of the cells hESCs on feeder-free culture systems tend to acquire senescence at earlier passages compared to those on feeder co-culture systems, and senescence is usually accompanied by morphological ... enzymatic passaging techniques and MEFconditioned medium [4] According to mTeSR-1’s manufacturers, StemCell Technologies, hESCs can be cultured on Matrigel in the defined culture medium for up to ... been reported to be able to proliferate for up to 100 passages, albeit under feeder co-culture systems [3, 4, 7] hESCs have also been reported to proliferate for up to 20 passages on Matrigel-coated...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
Ngày tải lên :
11/09/2015, 10:07
... into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell Bank In this protocol, the hESCs formed embryoid bodies using non-adherent cell culture ... little is known about the epigenetic factors that define the differentiation state or potency of stem cells in comparison to differentiated cells, DNA methylation studies were done The goal was...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
Ngày tải lên :
11/09/2015, 10:07
... developing an alternative embryonic stem cell culture system, with the aim of overcoming current obstacles that hinder the safe clinical implementation of human embryonic stem cell culture technologies ... signatures of seven individual uman embryonic stem cell lines Stem Cells 23:1343-1356 (2005) Stewart R, Stojkovic M, Lako M Mechanisms of self-renewal in human embryonic stem cells Eur J Cancer 40:1257-1272 ... Significant refinements were made at each human embryonic stem cell culture step that can contribute to a safer and more efficient human embryonic stem cell culture: For the enhancement of collagenous...
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A human embryonic stem cell based model of trophoblast formation
Ngày tải lên :
11/09/2015, 16:07
... related factors 21 iv 1.2.4 Clinical relevance of placenta development 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 22 23 23 1.3.1.1 Derivation of mESC 23 1.3.1.2 Culture condition ... field of ART and later clinical complications related to the pregnancy 23 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 1.3.1.1 Derivation of mESC mESCs were first derived in 1981 {Martin, ... ethylenediaminotetraacetic acid EGF epidermal growth factor EOMES eomesodermin EPI epiblast EpiSC epiblast stem cell ESC embryonic stem cell EST expressed sequence taq EtBR ethidium bromide xiv ETV4 Ets variant gene...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
Ngày tải lên :
09/08/2014, 20:22
... pluripotency and embryonic stem cell fate in Down syndrome Am J Hum Genet 2008, 83:388-400 19 Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H: An efficient system to establish multiple embryonic ... KR: Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation Methods Mol Biol 2002, 185:127-156 Wobus AM, Boheler KR: Embryonic stem ... JM, Humphries RK, Helgason CD: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency Stem Cells 2005, 23:663-680 35 Munne S, Howles CM, Wells...
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Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
Ngày tải lên :
13/08/2014, 09:21
... derivation of human embryonic stem cells Stem cells 2005, 23:1228-1233 Moon SY, Park YB, Kim D-S, Oh SK, Kim D-W: Generation, culture, and differentiation of human embryonic stem cells for therapeutic ... differentiation ofhuman embryonic stem cells Blood 2003, 102:906-915 Wang L, Li L, Menendez P, Cerdan C, Bhatia M: Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts ... derived from human embryonic stem cells exhibit an embryonic gene expression pattern Blood 2004, 103:4134-4141 Martin CH, Kaufman DS: Synergistic use of adult and embryonic stem cells to study...
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